The Tirang Beach area is a coastal area where there are many fish farming, including milkfish (Chanos chanos), shrimp and pufferfish (Gerres erythrourus). Aquaculture activities in this area are often hampered by bacterial infections that attack the bodies of farmed fish. The use of antibiotics to prevent disease is a growing trend, however it can lead to antibiotic resistance. Antibiotic resistance is a global problem that is important to research. This study aims to monitor the existence of resistant bacteria in sediment and fish from traditional pond as well as to identify the most resistant bacteria through molecular approach. The research was carried out from January to April 2024, with data collection using a random sampling method. Samples were taken from pond sediment and pond fish gills at three different points. Based on the results, the abundance of sediment and gill fish bacteria from traditional ponds in the Tirang Beach area has varied ranging from 1.53 x 106 to 2.45 x 107 CFU/ml and 7.2 x 105 to 5.52 x 106 CFU/ml, respectively. Furthermore, the resistance level of bacteria against Tetracycline, Erythromycin, Chloramphenicol, Ciprofloxacin obtained 36.67; 6.67; 30.00; and 56.67 percentage. Therefore, antibiotic resistance tests showed that the highest resistance was to Ciprofloxacin. Even though the abundance bacteria from sediment higher than gill fish, however the resistance bacteria in gill fish until 82% greater than sediment. Moreover, the observation through 16S rRNA gene revealed that the most resistant fish bacteria was identified as Vibrio alginolyticus, which is a rod-shaped Gram-negative bacterium whereas, the most resistant bacteria in sediment was identified as Vibrio parahaemolyticus, which harmful bacterium as well-known become causative agent of Acute Hepatopancreatic Necrosis Disease (AHPND). Hence, in traditional ponds while commercial antibiotics are non-use, Vibrio spp, which resistant to several commercial antibiotics are still found.
Sukma, D., Sabdaningsih, A., Jati, O., & Ayuningrum, D. (2024). Detection of Resistant Bacteria through Molecular Identification from Traditional Ponds in Tirang Beach, Semarang, Indonesia. Advances in Environmental Technology, (), -. doi: 10.22104/aet.2024.6913.1909
MLA
Devi Endah Melati Sukma; Aninditia Sabdaningsih; Oktavianto Eko Jati; Diah Ayuningrum. "Detection of Resistant Bacteria through Molecular Identification from Traditional Ponds in Tirang Beach, Semarang, Indonesia". Advances in Environmental Technology, , , 2024, -. doi: 10.22104/aet.2024.6913.1909
HARVARD
Sukma, D., Sabdaningsih, A., Jati, O., Ayuningrum, D. (2024). 'Detection of Resistant Bacteria through Molecular Identification from Traditional Ponds in Tirang Beach, Semarang, Indonesia', Advances in Environmental Technology, (), pp. -. doi: 10.22104/aet.2024.6913.1909
VANCOUVER
Sukma, D., Sabdaningsih, A., Jati, O., Ayuningrum, D. Detection of Resistant Bacteria through Molecular Identification from Traditional Ponds in Tirang Beach, Semarang, Indonesia. Advances in Environmental Technology, 2024; (): -. doi: 10.22104/aet.2024.6913.1909
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