Nutrients removal from raw municipal wastewater using Chlorella vulgaris microalgae

Document Type : Research Paper

Authors

1 Department of Chemical Engineering, University of Sistan and Baluchestan, Zahedan, Iran

2 Department of Chemical Technologies, Iranian Research Organization for Science and Technology, Tehran, Iran

3 Department of Biotechnology, Iranian Research Organization for Science and Technology, Tehran, Iran

Abstract

This study evaluated the ability of Chlorella vulgaris, a freshwater microalgae species, to remove nutrients from raw municipal wastewater. The wastewater was collected from the initial sedimentation-stage discharge of the treatment plant and used to cultivate the microalgae in both a shaker-incubator and a photobioreactor. The results showed that the microalgae effectively reduced the nitrate, nitrite, phosphate, and ammonium ion concentrations in the wastewater by over 90%. Phosphate removal was particularly efficient in the photobioreactor, with a removal rate of 91%, while the shaker- incubator had a removal rate of 44%. In addition to removing nutrients, the microalgae were also able to significantly reduce the wastewater’s chemical oxygen demand (COD), with a reduction of over 90% from 264 to 23.1 mg/l. The microalgae also had a symbiotic effect on the bacterial colonies present in the wastewater, reducing their numbers by 99% while allowing the microalgae to thrive. The final biomass concentration in the photobioreactor was 2.03 g/l, a higher value compared to similar studies. These results demonstrate the potential of Chlorella vulgaris and other microalgae species for use in wastewater treatment systems.

Graphical Abstract

Nutrients removal from raw municipal wastewater using Chlorella vulgaris microalgae

Keywords

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References

[1]  Spennati, E., Mirizadeh. S., Casazza, A. A., Solisio, C., Converti, A. ( 2021). Chlorella vulgaris and Arthrospira platensis growth in a continuous membrane photobioreactor using industrial winery wastewater. Algal research, 60, 102519.
[2]  Zhang, E., Wang, B., Wang, Q., Zhang, S., Zhao, B. (2008). Ammonia–nitrogen and orthophosphate removal by immobilized Scenedesmus sp. isolated from municipal wastewater for potential use in tertiary treatment. Bioresource technology, 99(9), 3787-93.
 [3]  Gonçalves, A. L., Pires, J. C., Simões, M. (2017). A review on the use of microalgal consortia for wastewater treatment. Algal research, 24, 403-15.
[4]  Benítez, M. B., Champagne, P., Ramos, A., Torres, A. F., Ochoa-Herrera, V.( 2019). Wastewater treatment for nutrient removal with Ecuadorian native microalgae. Environmental technology, 40(22), 2977-85.
[5]  Torres-Franco, A., Passos,F., Figueredo, C., Mota, C., Muñoz, R. (2021). Current advances in microalgae-based treatment of high-strength wastewaters: challenges and opportunities to enhance wastewater treatment performance. Reviews in environmental science and biotechnology, 20(1), 209-35.
[6] Otondo, A., Kokabian, B., Stuart-Dahl, S., Gude, V. G. (2018). Energetic evaluation of wastewater treatment using microalgae, Chlorella vulgaris. Journal of environmental chemical engineering, 6(2), 3213-22.
[7] Wang, L., Liu, J., Zhao, Q., Wei, W., Sun, Y.( 2016). Comparative study of wastewater treatment and nutrient recycle via activated sludge, microalgae and combination systems. Bioresource technology, 211, 1-5.
[8] Satpal, S., Khambete, A. K. (2016). Wastewater treatment using micro-algae -A review paper. International journal of engineering technology management and applied sciences, 4(2), 188-192.
[9] Al-Jabri, H., Das, P., Khan, S., Thaher, M., AbdulQuadir, M. (2021). Treatment of wastewaters by microalgae and the potential applications of the produced biomass-A review. Water, 13(1), 27.
[10] Yadav, G., Dash, S.K., Sen, R. (2019). A biorefinery for valorization of industrial wastewater and flue gas by microalgae for waste mitigation, carbon-dioxide sequestration and algal biomass production. Science of the total environment, 688,129-35.
[11] Ziganshina, E.  E., Bulynina, S. S., Ziganshin, A. M. (2021). Assessment of Chlorella sorokiniana growth in anaerobic digester effluent. Plants (Basel), 10(3), 478    
[12] Li, K., Liu, Q., Fang, F., Luo, R., Lu ,Q,. Zhou, W., Huo, Shuhao, Cheng, p., Liu, J., Addy, M., Chen, P., Chen, D., Ruan, R. ( 2019). Microalgae-based wastewater treatment for nutrients recovery: A review. Bioresource technology, 291, 121934.
[13] Kim, J., Lingaraju, B. P., Rheaume, R., Lee, J. Y., Siddiqui, K. F. (2010). Removal of ammonia from wastewater effluent by Chlorella vulgaris. Tsinghua science and technology, 15(4), 391-6.
[14] Chen, Z., Xiao, Y., Liu, T., Yuan, M., Liu, G., Fang, J., Yang, Bo. (2021). Exploration of microalgal species for nutrient removal from anaerobically digested swine wastewater and potential lipids production. Microorganisms, 9(12), 2469.
[15] Wang, L., Min, M., Li, Y., Chen, P., Chen, Y., Liu, Y., Wang, Y., Ruan, R. (2010). Cultivation of green algae Chlorella sp. in different wastewaters from municipal wastewater treatment plant. Applied biochemistry and biotechnology, 162(4), 1174-1186.
[16] Kim, J., Liu, Z., Lee, J-Y., Lu, T. (2013). Removal of nitrogen and phosphorus from municipal wastewater effluent using Chlorella vulgaris and its growth kinetics. Desalination and water treatment, 51(40-42), 7800-7806.
[17] Min, M., Wang, L., Li, Y., Mohr, M. J., Hu, B., Zhou, W., Zhou, W., Chen, P., Ruan, R., Micheal, J. (2011). Cultivating Chlorella sp. in a pilot-scale photobioreactor using centrate wastewater for microalgae biomass production and wastewater nutrient removal. Applied biochemistry and biotechnology, 165(1), 123-137.
[18] Ahmad, F., Khan, A. U., Yasar, A. (2013). The potential of Chlorella vulgaris for wastewater treatment and biodiesel production. Pakistan journal of botany, 45, 461-465.
[19] Gao, F., Yang, Z. H., Li, C., Zeng, G. M., Ma D-H, Zhou, L. (2015). A novel algal biofilm membrane photobioreactor for attached microalgae growth and nutrients removal from secondary effluent. Bioresource technology, 179, 8-12.
[20] Moondra, N., Jariwala, N. D., Christian, R. A. (2020). Sustainable treatment of domestic wastewater through microalgae. International journal of phytoremediation, 22(14), 1480-1486.        
[21] Rada-Ariza, A. M., Lopez-Vazquez, C. M., van der Steen, N., Lens, P. (2017). Nitrification by microalgal-bacterial consortia for ammonium removal in flat panel sequencing batch photo-bioreactors. Bioresource technology, 245, 81-89.
[22] Aditya, L., Mahlia, T. M. I., Nguyen, L. N., Vu, H. P., Nghiem, L. D. (2022). Microalgae-bacteria consortium for wastewater treatment and biomass production. Science of the total environment, 838, 155871.
[23] Fito, J., Alemu, K. (2018). Microalgae–bacteria consortium treatment technology for municipal wastewater management. Nanotechnology for environmental engineering, 4(1), 4.
[24] Ji, B., Liu, Y. (2021). Assessment of microalgal-bacterial granular sludge process for environmentally sustainable municipal wastewater treatment. ACS EST Water, 1(12), 2459-2469.
[25] Foladori, P., Petrini, S., Andreottola, G. (2018). Evolution of real municipal wastewater treatment in photobioreactors and microalgae-bacteria consortia using real-time parameters. Chemical engineering journal, 345, 507-516.
[26] Krustok, I., Odlare, M., Truu, J., Nehrenheim, E. (2016). Inhibition of nitrification in municipal wastewater-treating photobioreactors: Effect on algal growth and nutrient uptake. Bioresource technology, 202, 238-243.
[27] Su, Y., Mennerich, A., Urban, B. (2011). Municipal wastewater treatment and biomass accumulation with a wastewater-born and settleable algal-bacterial culture. Water research, 45(11), 3351-3358.
[28] Khaldi, H., Maatoug, M., Dube, C.S., Ncube, M., Tandlich, R., Heilmeier, H., Della, A., Laubscher, R. K.( 2017). Efficiency of wastewater treatment by a mixture of sludge and microalgae. Journal of fundamental and applied sciences, 9(3), 1454-1572.
[29] Jiang, H., Luo, S., Shi, X., Dai, M., Guo, R.B. ( 2012). A novel microbial fuel cell and photobioreactor system for continuous domestic wastewater treatment and bioelectricity generation. Biotechnology letters, 34(7), 1269-1274.
[30] Boonchai, R., Seo, G., Park, D., Seong, C. (2012). Microalgae photobioreactor for nitrogen and phosphorus removal from wastewater of sewage treatment plant. International journal of bioscience, biochemistry and bioinformatics, 2, 407-410.
[31] Sukačová, K., Trtílek, M., Rataj, T. ( 2015). Phosphorus removal using a microalgal biofilm in a new biofilm photobioreactor for tertiary wastewater treatment. Water research, 71, 55-63.
[32] Singh, P., Singh, M. K., Beg, Y. R., Nishad, G. R. (2019). A review on spectroscopic methods for determination of nitrite and nitrate in environmental samples. Talanta, 191, 364-381.
[33]  Dickman, S. R., Bray, R. H. (1940). Colorimetric determination of phosphate. Industrial and engineering chemistry analytical edition, 12(11), 665-8.
[34] Posadas, E., Alcántara, C., García-Encina, P. A., Gouveia, L., Guieysse, B., Norvill, Z., Muñoz, R. (2017). Microalgae cultivation in wastewater. Microalgae-based biofuels and bioproducts: From feedstock cultivation to end-products, 1st. Edition, Elsevier Pub., 67–91.
[35] Arias, D. M., Solé-Bundó, M., Garfí, M., Ferrer, I., García, J., Uggetti, E. (2018). Integrating microalgae tertiary treatment into activated sludge systems for energy and nutrients recovery from wastewater. Bioresource technology, 247, 513-519.      
[36] Ponte, W. M. L., Talaverano, N. Z., Huaynate, A., O., Cafferata, E. A., Gallegos, M. C. (2022). Efficiency of microalgae cultures for nutrient removal from domestic wastewater. Advances in environmental technology, 8(1), 73-81.
[37] Rani, S., Chowdhury, R., Tao, W., Nedbalová, L., Saha, K. (2021). Microalga-mediated tertiary treatment of municipal wastewater: Removal of nutrients and pathogens. Sustainability, 13, 9554.
Advances in Environmental Technology
Volume 9, Issue 1
January 2023
Pages 47-57

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