Response surface methodology approach for simultaneous carbon, nitrogen, and phosphorus removal from industrial wastewater in a sequencing batch reactor

Document Type : Research Paper


1 Department of Biology, Islamic Azad University, Tonekabon Branch, Tonekabon, Mazandaran, Iran

2 Department of Environmental Science, University of Jiroft, Jiroft, Iran

3 Department of Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran

4 Discipline of Chemical Engineering, School of Engineering, Monash University Malaysia, Jalan Lagoon Selatan, Bandar Sunway, 47500 Subang Jaya, Selangor Malaysia

5 School of Science, Monash University Malaysia, Jalan Lagoon Selatan, Bandar Sunway, 47500 Subang Jaya, Selangor Malaysia

6 Department of Environmental Science, Tarbiat Modares University, Noor, Iran

7 Water and Wastewater Research Center (WWRC), Razi University, Kermanshah, Iran


Wastewater reclamation involving a sequencing batch reactor (SBR) has received more attention recently due to its high nutrient removal efficiency, cost-effectiveness, and low footprint. This study attempts to develop a stable and applicable activated sludge SBR for simultaneous carbon and nutrient removal from industrial wastewater. The derived-filed data were explored by response surface methodology (RSM) to identify the impact of operational variables on the SBR performance. Optimum conditions were obtained at 4000 mg/L MLSS, 100: 8: 2 COD: N: P ratio, 40 min/h aeration time, and 40 h cycling time, which resulted in the removal of 82.53% chemical oxygen demand (COD), 89.83% TKN, 87.23% PO43--P, and 73.46% NO3--N. Moreover, the sludge volume index (SVI) and mixed liquor volatile suspended solids (MLVSS)/mixed liquor suspended solids (MLSS) ratio were 64.8 mL/g and 0.8, respectively. The maximum nitrification rate was calculated as 113.9 mg/L.d, which increased with the rise of the initial ammonium concentration. The specific denitrification rate (SDNR) was estimated in the range of 0.003-0.07 mgNO3--N/mg MLVSS.d, depicting the high potential of the SBR reactor to eliminate nitrate by granular sludge. Accordingly, the removal efficiency of the optimized system revealed a notable capability towards meeting environmental regulations. 


Main Subjects

[1] Amini, M., Khoei, Z. A., Erfanifar, E. (2019). Nitrate (NO3) and phosphate (PO43−) removal from aqueous solutions by microalgae Dunaliella salina. Biocatalysis and agricultural biotechnology, 19, 101097.
[2] Moradi, S., Zinatizadeh, A., Zinadini, S., Gholami, F. (2021). High-rate CNP removal from wastewater in a single jet loop air lift bioreactor: Process modeling and optimization with four process and operating factors. Journal of water process engineering, 40, 101980.
[3] Nguyen, T. T., Némery, J., Gratiot, N., Strady, E., Tran, V. Q., Nguyen, A. T., Aimé, J., Peyne, A. (2019). Nutrient dynamics and eutrophication assessment in the tropical river system of Saigon–Dongnai (southern Vietnam). Science of the total environment, 653, 370-383.
[4] Watsuntorn, W., Ruangchainikom, C., Rene, E. R., Lens, P. N., Chulalaksananukul, W. (2019). Comparison of sulphide and nitrate removal from synthetic wastewater by pure and mixed cultures of nitrate-reducing, sulphide-oxidizing bacteria. Bioresource technology, 272, 40-47.
[5] Crini, G., Lichtfouse, E. (2019). Advantages and disadvantages of techniques used for wastewater treatment. Environmental chemistry letters, 17(1), 145-155.
[6] Fayazi, M. (2020). Removal of mercury (II) from wastewater using a new and effective composite: sulfur-coated magnetic carbon nanotubes. Environmental science and pollution research, 27(11), 12270-12279.
[7] Fayazi, M., Ghanbarian, M. One-pot hydrothermal synthesis of polyethylenimine functionalized magnetic clay for efficient removal of noxious Cr (VI) from aqueous solutions. Silicon, 12(1), 125-134.
[8] Khaki, E., Abyar, H., Nowrouzi, M., Younesi, H., Abdollahi, M., Enderati, M. G. (2021). Comparative life cycle assessment of polymeric membranes: Polyacrylonitrile, polyvinylimidazole and poly (acrylonitrile-co-vinylimidazole) applied for CO2 sequestration. Environmental technology and innovation, 22, 101507.
[9] Abyar, H., Younesi, H., Bahramifar, N., Zinatizadeh, A. A. (2018). Biological CNP removal from meat-processing wastewater in an innovative high rate up-flow A2O bioreactor. Chemosphere, 213, 197-204.
[10] Li, J., Wu, B., Li, Q., Zou, Y., Cheng, Z., Sun, X., Xi, B. (2019). Ex situ simultaneous nitrification-denitrification and in situ denitrification process for the treatment of landfill leachates. Waste management, 88, 301-308.
[11] Lei, X., Jia, Y., Chen, Y., Hu, Y. (2019). Simultaneous nitrification and denitrification without nitrite accumulation by a novel isolated Ochrobactrum anthropic LJ81. Bioresource technology, 272, 442-450.
[12] Iorhemen, O. T., Hamza, R. A., Zaghloul, M. S., Tay, J. H. (2019). Aerobic granular sludge membrane bioreactor (AGMBR): Extracellular polymeric substances (EPS) analysis. Water research, 156, 305-314.
[13] Lotti, T., Carretti, E., Berti, D., Martina, M. R., Lubello, C., Malpei, F. (2019). Extraction, recovery and characterization of structural extracellular polymeric substances from anammox granular sludge. Journal of environmental management, 236, 649-656.
[14] Soler-Jofra, A., Wang, R., Kleerebezem, R., van Loosdrecht, M. C., Pérez, J. (2019). Stratification of nitrifier guilds in granular sludge in relation to nitritation. Water research, 148, 479-491.
[15] Cerruti, M., Stevens, B., Ebrahimi, S., Alloul, A., Vlaeminck, S. E., Weissbrodt, D. G. (2020). Enrichment and aggregation of purple non-sulfur bacteria in a mixed-culture sequencing-batch photobioreactor for biological nutrient removal from wastewater. Frontiers in bioengineering and biotechnology, 8, 557234. doi: 10.3389/fbioe.2020.557234.
[16] Desireddy, S., Sabumon, P. (2021). Development of aerobic granulation system for simultaneous removal of C, N, and P in sequencing batch airlift reactor. Journal of Environmental chemical engineering, 9(5), 106100.
[17] Fayazi, M., Afzali, D., Taher, M., Mostafavi, A., Gupta, V. (2015). Removal of Safranin dye from aqueous solution using magnetic mesoporous clay: Optimization study. Journal of molecular lquids, 212, 675-685.
[18] Nowrouzi, M., Abyar, H. (2021). A framework for the design and optimization of integrated fixed-film activated sludge-membrane bioreactor configuration by focusing on cost-coupled life cycle assessment. Journal of cleaner production, 296, 126557.
[19] APHA, 1999. Standard methods for the examination of water and wastewater, American public health association, Washington, DC.
[20] Fan, J., Ye, J., Zhang, H., Ji, B., Ye, W. (2018). Integrated Effect of MLSS and SRT on Performance of a full-scale modified A2/O Process. Polish journal of environmental studies, 27(4), 1475-1482
[21] Hauduc, H., Al-Omari, A., Wett, B., Jimenez, J., De Clippeleir, H., Rahman, A., Wadhawan, T., Takacs, I. (2019). Colloids, flocculation and carbon capture–a comprehensive plant-wide model. Water science and technology, 79(1), 15-25.
[22] He, Q., Zhang, S., Zou, Z., Zheng, L., Wang, H. (2016). Unraveling characteristics of simultaneous nitrification, denitrification and phosphorus removal (SNDPR) in an aerobic granular sequencing batch reactor. Bioresource technology, 220, 651-655.
[23] Amini, M., Younesi, H., Najafpour, G., Zinatizadeh-Lorestani, A. A. (2012). Application of response surface methodology for simultaneous carbon and nitrogen (SND) removal from dairy wastewater in batch systems. International journal of environmental studies, 69(6), 962-986,
[24] Xu, H., Liu, Y., Gao, Y., Li, F., Yang, B., Wang, M., Ma, C., Tian, Q., Song, X., Sand, W. (2018). Granulation process in an expanded granular sludge blanket (EGSB) reactor for domestic sewage treatment: Impact of extracellular polymeric substances compositions and evolution of microbial population. Bioresource technology, 269, 153-161.
[25] Hayati, H., Doosti, M., Sayadi, M. (2013). Performance evaluation of waste stabilization pond in Birjand, Iran for the treatment of municipal sewage. Proceedings of the international academy of ecology and environmental sciences, 3(1), 52-58.
[26] He, Q., Zhang, W., Zhang, S., Wang, H. (2017). Enhanced nitrogen removal in an aerobic granular sequencing batch reactor performing simultaneous nitrification, endogenous denitrification and phosphorus removal with low superficial gas velocity. Chemical engineering journal, 326, 1223-1231.
[27] Zhang, L., Huang, Y., Li, S., He, P., Wang, D. (2018). Optimization of nitrogen removal in solid carbon source SND for treatment of low-carbon municipal wastewater with RSM method. Water, 10(7), 827. doi:10.3390/w10070827.
[28] He, Q., Chen, L., Zhang, S., Wang, L., Liang, J., Xia, W., Wang, H., Zhou, J. (2018). Simultaneous nitrification, denitrification and phosphorus removal in aerobic granular sequencing batch reactors with high aeration intensity: Impact of aeration time. Bioresource technology, 263, 214-222.
[29] Li, J., Meng, J., Li, J., Wang, C., Deng, K., Sun, K., Buelna, G. (2016). The effect and biological mechanism of COD/TN ratio on nitrogen removal in a novel upflow microaerobic sludge reactor treating manure-free piggery wastewater. Bioresource technology, 209, 360-368.
[30] Mannina, G., Capodici, M., Cosenza, A., Di Trapani, D., van Loosdrecht, M. C. (2017). Nitrous oxide emission in a University of Cape Town membrane bioreactor: the effect of carbon to nitrogen ratio. Journal of cleaner production, 149, 180-190.
[31] Akhbari, A., Zinatizadeh, A., Mohammadi, P., Irandoust, M., Mansouri, Y. (2011). Process modeling and analysis of biological nutrients removal in an integrated RBC-AS system using response surface methodology. Chemical engineering journal, 168(1), 269-279.
[32] Zou, J., Pan, J., Wu, S., Qian, M., He, Z., Wang, B., Li, J. (2019). Rapid control of activated sludge bulking and simultaneous acceleration of aerobic granulation by adding intact aerobic granular sludge. Science of the total environment, 674, 105-113.
[33] Salehi, S., Cheng, K. Y., Heitz, A., Ginige, M. P. (2019). A novel storage driven granular post denitrification process: Long-term effects of volume reduction on phosphate recovery. Chemical engineering journal, 356, 534-542.
[34] Onetto, C. A., Eales, K. L., Guagliardo, P., Kilburn, M. R., Gambetta, J. M., Grbin, P. R. (2017). Managing the excessive proliferation of glycogen accumulating organisms in industrial activated sludge by nitrogen supplementation: A FISH-NanoSIMS approach. Systematic and applied microbiology, 40, 500-507.
[35] Sayadi, M., Ahmadpour, N., Fallahi, C. M., Rezaei, M. (2016). Removal of nitrate and phosphate from aqueous solutions by microalgae: An experimental study. Global Journal of environmental science and management, 2(4), 357-364.
[36] Badia, A., Kim, M., Nakhla, G., Ray, M. B. (2019). Effect of COD/N ratio on denitrification from nitrite. Water environment research, 91(2), 119-131
[37] Ge, S., Peng, Y., Wang, S., Lu, C., Cao, X., Zhu, Y. (2012). Nitrite accumulation under constant temperature in anoxic denitrification process: The effects of carbon sources and COD/NO3-N. Bioresource technology, 114, 137-143.
[38] Vjayan, T., Vadivelu, V. (2017). Effect of famine-phase reduced aeration on polyhydroxyalkanoate accumulation in aerobic granules. Bioresource technology, 245, 970-976.
[39] Aziz, S. Q., Aziz, H. A., Yusoff, M. S., Bashir, M. J. (2011). Landfill leachate treatment using powdered activated carbon augmented sequencing batch reactor (SBR) process: Optimization by response surface methodology. Journal of hazardous materials, 189(1-2), 404-413.
[40] Fan, J., Ji, F., Xu, X., Wang, Y., Yan, D., Xu, X., Chen, Q., Xiong, J., He, Q. (2015). Prediction of the effect of fine grit on the MLVSS/MLSS ratio of activated sludge. Bioresource technology, 190, 51-56.
[41] Zhang, J., Shao, Y., Wang, H., Liu, G., Qi, L., Xu, X., Liu, S. (2021). Current operation state of wastewater treatment plants in urban China. Environmental research, 195, 110843.
[42] Kraus, T. E., O'Donnell, K., Downing, B. D., Burau, J. R., Bergamaschi, B. (2017). Using paired in situ high frequency nitrate measurements to better understand controls on nitrate concentrations and estimate nitrification rates in a wastewater‐impacted river. Water resources research, 53(10), 8423-8442.
[43] Debik, E., Manav, N. (2010). Sequence optimization in a sequencing batch reactor for biological nutrient removal from domestic wastewater. Bioprocess and biosystems engineering, 33(5), 533-540.
[44] Ribera-Guardia, A., Marques, R., Arangio, C., Carvalheira, M., Oehmen, A., Pijuan, M. (2016). Distinctive denitrifying capabilities lead to differences in N2O production by denitrifying polyphosphate accumulating organisms and denitrifying glycogen accumulating organisms. Bioresource technology, 219, 106-113.
[45] Wang, S., Deng, L., Zheng, D., Wang, L., Zhang, Y., Yang, H., Jiang, Y., Huang, F. (2018). Control of partial nitrification using pulse aeration for treating digested effluent of swine wastewater. Bioresource technology, 262, 271-277.
[46] Ge, S., Wang, S., Yang, X., Qiu, S., Li, B., Peng, Y. (2015). Detection of nitrifiers and evaluation of partial nitrification for wastewater treatment: a review. Chemosphere, 140, 85-98.